![]() The use of molecular methods in bacteriology has resulted in increased knowledge about biodiversity within the genus Campylobacter. At present the genus Campylobacter contains 17 species, four of which have been further divided into eight subspecies. The genus Campylobacter was introduced by Sebald and Verón and its taxonomic structure has been revised a number of times. Campylobacter jejuni is frequently found in the intestinal tract in a wide variety of wild and domesticated animals, especially poultry. In the present paper Campylobacter jejuni subsp. jejuni, are important food- and water-borne pathogens for man. A potential correlation was found between the Sma I profiles and the 16S rRNA sequences, as a certain Sma I type only appeared in one of the two major phylogenetic groups.Ĭampylobacter spp., principally Campylobacter jejuni subsp. Genotyping of the 47 strains by PFGE after digestion with Sma I resulted in 22 subtypes. The strains were grouped in two major clusters according to 16S rRNA, one cluster with only C. coli seem to lack polymorphisms in their 16S rRNA gene, but phylogenetic analysis based on 16S rRNA sequences was not always sufficient for differentiation between C. The three 16S rRNA genes in the analysed strains were identical within each individual strain for all 47 strains. Five of the nine sequence types were also found among the Campylobacter sequences deposited in GenBank. The other 10 strains were identified as Campylobacter jejuni. One of the nine 16S rRNA sequence profiles was common to 12 of the strains from our study and two of these were identified as Campylobacter coli by PCR/REA. The number of nucleotide substitutions varied between one and six among the nine 16S rRNA sequence types. Sequence analyses of the 16S rRNA genes revealed nine sequence types of the Campylobacter strains and the similarities between the different sequence types were in the range 99.6–99.9%. The strains were also genotyped by PFGE after digestion with Sma I. coli strains isolated from broilers were sequenced and compared with 16S rRNA sequences retrieved from the Ribosomal Database Project or GenBank. coli and to determine whether the 16S rRNA sequence types correlated with genotypes generated by PFGE analysis of Sma I restricted genomic DNA of the strains. The objectives in this study were to determine the degree of intraspecific variation in the 16S rRNA genes of C. Sequence analysis of the 16S rRNA gene is very useful for identification of bacteria to genus and species level. predominantly found in infected humans and colonized broilers. Campylobacter jejuni is the Campylobacter sp. One significant source of infection is broilers and consumption of undercooked broiler meat. Campylobacter is the most commonly reported bacterial cause of enteritis in humans in the EU Member States and other industrialized countries.
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